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psgl 1 fc  (R&D Systems)


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    R&D Systems psgl 1 fc
    Psgl 1 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+psgl/Recombinant+Human+PSGL-1%2FCD162+Fc+Chimera+Protein%2C+CF/pm41203159-54-12-25
    Average 93 stars, based on 17 article reviews
    psgl 1 fc - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Serial Dilution:

    Article Title: Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
    Article Snippet: *Corresponding author.. School of B University of Auckland, Auckland, E-mail address: ted.baker@auckland Abbreviations used: CHIPS, chem protein of Staphylococcus aureus; FAC fluorescence-activated cell sorting; M histocompatibility complex; NAG, N OB, oligonucleotide/oligosaccharide protein data bank; PEG, polyethylen P-selectin glycoprotein ligand-1; PT, superantigen; Sia, sialic acid; sLe, s staphylococcal superantigen-like pro receptor.

    Recombinant:

    Article Title: Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
    Article Snippet: *Corresponding author.. School of B University of Auckland, Auckland, E-mail address: ted.baker@auckland Abbreviations used: CHIPS, chem protein of Staphylococcus aureus; FAC fluorescence-activated cell sorting; M histocompatibility complex; NAG, N OB, oligonucleotide/oligosaccharide protein data bank; PEG, polyethylen P-selectin glycoprotein ligand-1; PT, superantigen; Sia, sialic acid; sLe, s staphylococcal superantigen-like pro receptor.

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Article Title: Pterostilbene Exhibits Broad-Spectrum Antiviral Activity by Targeting the Enterovirus Capsid, Inactivating Viral Particles, Blocking Viral Binding, and Protecting Mice From Lethal EV-A71 Challenge.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 17 Phytotherapy Research, 2025 incubated with 1 μg of recombinant human- PSGL- 1- Fc (3345- PS; R&D Systems, Minneapolis, MO, USA), human- SCARB2- Fc (1966- LM; R&D Systems), or human- ICAM- 5- Fc (1950- M5; R&D Systems) protein for 1 h at 4°C. ..

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2.
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Produced:

    Article Title: Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
    Article Snippet: *Corresponding author.. School of B University of Auckland, Auckland, E-mail address: ted.baker@auckland Abbreviations used: CHIPS, chem protein of Staphylococcus aureus; FAC fluorescence-activated cell sorting; M histocompatibility complex; NAG, N OB, oligonucleotide/oligosaccharide protein data bank; PEG, polyethylen P-selectin glycoprotein ligand-1; PT, superantigen; Sia, sialic acid; sLe, s staphylococcal superantigen-like pro receptor.

    Transfection:

    Article Title: Crystal structures of the staphylococcal toxin SSL5 in complex with sialyl Lewis X reveal a conserved binding site that shares common features with viral and bacterial sialic acid binding proteins.
    Article Snippet: *Corresponding author.. School of B University of Auckland, Auckland, E-mail address: ted.baker@auckland Abbreviations used: CHIPS, chem protein of Staphylococcus aureus; FAC fluorescence-activated cell sorting; M histocompatibility complex; NAG, N OB, oligonucleotide/oligosaccharide protein data bank; PEG, polyethylen P-selectin glycoprotein ligand-1; PT, superantigen; Sia, sialic acid; sLe, s staphylococcal superantigen-like pro receptor.

    Incubation:

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Article Title: Pterostilbene Exhibits Broad-Spectrum Antiviral Activity by Targeting the Enterovirus Capsid, Inactivating Viral Particles, Blocking Viral Binding, and Protecting Mice From Lethal EV-A71 Challenge.
    Article Snippet: .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 17 Phytotherapy Research, 2025 incubated with 1 μg of recombinant human- PSGL- 1- Fc (3345- PS; R&D Systems, Minneapolis, MO, USA), human- SCARB2- Fc (1966- LM; R&D Systems), or human- ICAM- 5- Fc (1950- M5; R&D Systems) protein for 1 h at 4°C. ..

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2.
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Bacteria:

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Article Title: Characterization of the zinc metalloprotease of Streptococcus suis serotype 2.
    Article Snippet: .. Briefly, 10 ng of recombinant human PSGL-1/CD162 Fc (R&D Systems, Minneapolis, MN, USA) were incubated with recombinant Zmp (100 μg), S. suis or S. pneumoniae (washed bacteria and supernatants, as described above) for 1 h at 37 °C. .. Samples were then separated on 7.5% SDS-PAGE under denaturing conditions and transferred to polyvinyl difluoride (PVDF) membrane (EMD Millipore).

    Sandwich ELISA:

    Article Title: Exploring dysregulated immune response genes and endothelial dysfunction biomarkers as predictors of severe COVID-19.
    Article Snippet: Identifying individuals and factors associated with severe cases of COVID-19 is crucial as the pandemic continues to spread globally.. Effective biomarkers for predicting severe cases are essential for optimizing clinical management, therapy, and preventing unfavorable outcomes.. This exploratory observational study aimed to investigate the expression of dysregulated immune response genes (ARG1, NOS2, ITGA4, and SELPLG) in total leukocytes, plasmatic levels of P-selectin and PSGL-1, and their clinical associations in patients with mild and severe COVID-19.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Exploring dysregulated immune response genes and endothelial dysfunction biomarkers as predictors of severe COVID-19.
    Article Snippet: Identifying individuals and factors associated with severe cases of COVID-19 is crucial as the pandemic continues to spread globally.. Effective biomarkers for predicting severe cases are essential for optimizing clinical management, therapy, and preventing unfavorable outcomes.. This exploratory observational study aimed to investigate the expression of dysregulated immune response genes (ARG1, NOS2, ITGA4, and SELPLG) in total leukocytes, plasmatic levels of P-selectin and PSGL-1, and their clinical associations in patients with mild and severe COVID-19.



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    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 <t>(PSGL-1)</t> residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.
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    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 <t>(PSGL-1)</t> residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.
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    Image Search Results


    Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.

    Journal: The Journal of Biological Chemistry

    Article Title: The protease cathepsin K can debulk the cancer glycocalyx

    doi: 10.1016/j.jbc.2026.111206

    Figure Lengend Snippet: Cathepsin K (CTSK) tolerates glycans near the cleavage site. A, cleavage motif of CTSK was generated from mass spectrometry analysis of ( left ) glycopeptides, ( center ) nonmodified peptides, and ( right ) modified and nonmodified peptides generated from CTSK digestion of purified and recombinant mucins and nonmucin glycoproteins, followed by trypsin digestion (see the section). The bar graphs on top of the glycopeptide cleavage motif indicate the frequency of O -glycosylation at each threonine and serine residue at that position. B, top, visualization of CTSK cleavage sites in recombinant MUC1 residues 24–47. The sialylated core-1 glycan at specific resides indicates that glycans were seen at those sites. This specific glycan was seen often in the dataset, but its depiction here is only intended to indicate glycosites, not to represent the diversity of all glycans detected at each glycosite in the dataset. Purple diamond , sialic acid; yellow circle , galactose; yellow square , N-acetylgalactosamine; and yellow circle , glycosylation site. Colored bars represent individual detected peptide sequences from CTSK cleavage only, with any shared peptides with chymotrypsin removed. Bottom, annotated spectrum for the indicated MUC1 O -glycopeptide. C, top, visualization of CTSK cleavage sites in recombinant P-selectin glycoprotein ligand-1 (PSGL-1) residues 148 to 197, represented as in ( B ), but this time from the tryptic + CTSK dataset, with all tryptic cleavage sites removed. Bottom, annotated spectrum for the indicated PSGL-1 O -glycopeptide. MUC, mucin.

    Article Snippet: Purified recombinant PSGL-1 (Sino Biological; 13863-H08H) was labeled with IRDye 800CW NHS Ester (LI-COR Biosciences) following the manufacturer’s recommendations.

    Techniques: Generated, Mass Spectrometry, Modification, Purification, Recombinant, Glycoproteomics, Residue